The 33-year-old former England captain was spotted drinking with Vicki Rosiek until 2.30am last weekend in Clearwater Beach, Florida.
Wife Coleen was said to have torn into the Wayne, telling him: 'You've humiliated me again!'
'Coleen has never been angrier at Wayne,' a source told the newspaper.
Rooney pictured with the barmaid in a bar in Clearwater, Florida last week during a ten-hour drinking session
The 33-year-old former England captain was spotted drinking with Vicki Rosiek until 2.30am last weekend
Rosiek is a local barmaid who works in the Florida area, where Rooney was with his DC United teammates
'She was furious Wayne was back in the news for boozing and cannot believe he has been so stupid as to find himself caught up with another woman again. It's just so embarrassing.
The incident comes just weeks after he was arrested for being drunk at an airport in Washington DC.
It is the latest in a string of drinking incidents for the former Manchester United and Everton striker, including a court appearance for drink-driving in 2017.
The football star was also pictured withdrawing cash from an ATM outside a local Hooters
Rooney pictured leaving one of the bars on his eventful night out last weekend in Florida
Wayne Rooney is in trouble with wife Coleen after he got into a car alone with barmaid Vicki Rosiek
Rooney began his ten hour drinking session at 4.20pm and had been with his DC United teammates but then was seen separately with Ms Rosiek, 32, who he had met a few days earlier.
Earlier that evening, the DC United striker was seen using a cash machine outside a Hooters bar in Florida.
There is no suggestion anything happened between Rooney and Ms Rosiek.
Rooney's marriage was in jeopardy 18 months ago when he was arrested for driving party girl Laura Simpson, 30, in her VW Beetle in Cheshire while three times over the limit.
He was handed a two-year driving ban and 100 hours of unpaid community work, apologising for an 'unforgiveable lack of judgement'.
Coleen, then pregnant, and their other three sons, Kai, Klay and Kit, were on holiday in Spain at the time.
In December he was taken into custody at Washington's Dulles airport, saying he was left 'disorientated' by prescribed sleeping tablets he took on a flight while drinking.
He received a statutory automatic fine and was released shortly afterwards at the airport, a spokesman for Rooney said.
Rooney, pictured left in a police mugshot after he was arrested in Washington at the end of last year, could face a fresh row with his wife Coleen, pictured together right
In 2016 he was at the centre of another drinking row at a wedding reception between England games, later apologising 'unreservedly' for his behaviour.
It was reported in April 2017 that he had been seen drinking at least five pints of Stella at a Cheshire pub on a night out.
Rooney has been in Florida with his DC United teammates as they prepare for the new upcoming MLS season.
He was seen watching the Super Bowl at 4.20pm last Sunday at the Pier House 60 Marina hotel.
Along with teammates, he went from bar to bar and then went with another team mate to the Brown Boxer.
He had also been seen getting money out near a Hooters bar - a restaurant chain renowned for waitresses wearing tiny outfits while serving customers.
Rooney and his friend reportedly went to the Shipwreck bar (pictured) where the had drinks for a few hours
Speaking about her encounter with Rooney, Ms Rosiek said she did not know who Rooney until a colleague told her.
She said: 'He was on his own. When I saw him, I said, 'Oh hey, I waited on you before'. Then we hung out.
'There was a group of us there. I was sitting there, just bulls****ing with him. He seemed down to earth, just a regular dude a little shy and quiet.'
After talking for ten minutes at the Brown Boxer, Ms Rosiek offered Rooney, who had been drinking light beer Michelob Ultra, a lift to the next bar.
They left together and she drove them to The Shipwreck at 12.30am.
She said they 'hung out for a few hours' and felt Rooney 'wanted someone to talk to without talking about soccer'.
Rooney left the bar at 2.30am and then she left at 3am.
Rooney has four children with wife and childhood sweetheart Coleen (pictured together)
He tried to get DJ Matt Valet to play Mumford and Sons song Timshel, although couldn't make himself understood due to his thick accent.
DJ Matt Valet said: 'In the end, he had to type it into my phone. I'd never heard of the song and unfortunately it wasn't in my playlist. He seemed bummed out when I could not play it.
The bar where Rooney was drinking is well known for its lemon, rum and watermelon cocktail called Pink Pussy.
While Rooney was enjoying a night on the town, Coleeen was thought to be at home in Washington.
A source said: 'She will be fuming, especially as she feels she's sacrificed a lot to move to the States for him.'